ABSTRACT
Objective: Annexin A1 [ANXA1] is suggested to have anti-inflammatory function. However, the precise function of ANXA1 has remained unclear. In this study, we therefore examined the potency of ANXA1 in regulating reactive oxygen species [ROS] production and suppressing pro-inflammatory responses in PC12 cells induced by 1-methyl-4-phenylpyridinium [MPP+]
Materials and Methods: In this experimental study, cDNA of ANXA1 was cloned and inserted to the PGL268 pEpi-FGM18F vector to produce a recombinant PGL/ANXA1 vector for transfection into the PC12 cells. ANXA1 transfected cells were then treated with MPP+. Apoptosis and the content of pro-inflammatory factors including ROS, Interlukin-6 [IL-6], inducible nitric oxide synthase [iNOS] and nuclear factor-kappa B [NF-kappaB] were assessed by flow-cytometry, real-time quantitative polymerase chain reaction [RT-qPCR] and western blot in ANXA1-transfected cells and the data were compared with those obtained from mock and control cells
Results: Data revealed that overexpression of ANXA1 is associated with decreased levels of ROS and expression level of IL-6 and iNOS transcripts, and NF-kappaB protein in MPP+ treated PC12 cells
Conclusion: ANXA1 may be considered as an agent for prevention of neurodegenerative or inflammatory conditions